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Image Search Results
Journal: Frontiers in Cellular Neuroscience
Article Title: Interferon-γ augments GABA release in the developing neocortex via nitric oxide synthase/soluble guanylate cyclase and constrains network activity
doi: 10.3389/fncel.2022.913299
Figure Lengend Snippet: IFN-γ increased mIPSC frequencies when amplitude augmentation was suppressed by postsynaptic PKC inhibition via calphostin C. (A) Example traces of mIPSCs recorded under postsynaptic intracellular application of PKC inhibitor Calphostin C (100 nM) before ( black ) and with IFN-γ ( green ). (B) Under intracellular PKC inhibition, IFN-γ, bath applied for 25.0 ± 1.0 min, increased mIPSC frequency and shifted IEI probability distribution toward shorter IEIs ( p < 0.0001, Kolmogorov–Smirnov test, ctrl = 2,254 IEIs vs. IFN-γ = 3,359 IEIs, 8 recordings, N = 2). Mean amplitudes remained comparable, however, amplitude probability distribution was slightly shifted toward lower amplitudes (below 80%), as well as higher amplitudes (above 90%) ( p < 0.0001, Kolmogorov–Smirnov test, ctrl = 2,270 events vs. IFN-γ = 3,375 events, 8 recordings, N = 2). Series resistance slightly increased from R s CalC ctrl = 15.4 ± 1.9 MΩ to R s CalC IFN_–γ = 17.5 ± 2.2 MΩ ( p = 0.001, paired t -test, n = 8, N = 2), holding current slightly decreased from −196.9 ± 27.1 pA to −121.1 ± 11.2 pA ( p = 0.02, paired t -test, n = 8, N = 2). * p < 0.05.
Article Snippet:
Techniques: Inhibition